In vitro regeneration of Hypochaeris radicata L. from sodium alginate-encapsulated synthetic seeds

Jamuna Senguttuvan, Paulsamy Subramaniam


Synthetic seeds were produced from in vitro derived leaf, root and callus explants of Hypochaeris radicata (hairy cat’s-ear) by encapsulating different concentrations of sodium alginate hydrogel (1-6%) containing MS medium. The texture, conversion frequency and the effect of temperature on shoot emergence were evaluated. Among three explants attempted, in vitro derived leaf segments encapsulated beads stored at 25°C observed successful shoot regeneration above 80% in the standardized MS medium supplemented with 2mg/L BAP. The regenerated shoots were rooted well (73.54%) on MS medium with 1mg/L NAA. The high frequency of plant re-growth was found in four month old leaf segment encapsulated beads. Generally, four months stored beads of all explants at 25°C found to have higher regeneration rate in comparison with 2 and 6 months old encapsulated beads.

Full Text:



Abdel Hamid, M.H., Rosna Mat, T. and Sadegh, M. 2013. In vitro induction and proliferation of adventitious roots in pinapple (Ananas comosus L.) cultivars of smooth cayenne and morris. Aust J crop sci. 7(7): 1038-1045.

Aman, N. and Shruti, S. 2013. Production of artificial seeds from nodal region of sweet neem (Murraya Koenigii). J Adv Pharmaceu Res Biosci. 1(2): 71-74.

Anju, P., Suraj, P.V., Promila, P. and Lucky, K.A. 2012. Augmented shelf-life and regeneration competence of activated charcoal (AC) supplemented synthetic seeds in Cymbidium pendulum (Roxb.). Sw. Curr Bot. 3(5): 30-34.

Bapat, B.A. and Rao, P.S. 1998. Sandalwood plantlets from synthetic seeds. Plant Cell Rep. 7: 434-436.

Bekheet, S.A. 2006. A synthetic seed method through encapsulation of in vitro proliferation bulblets of garlic (Allium sativum L.). Arab J Biotech. 9(3): 415-426.

Block, W. 2003. Water status and thermal analysis of alginate beads used in cryopreservation of plant germplasm. Cryobiology 47(1): 59-72.

Dennis, J.G., Purohit, A. and Triglano, R.N. 1991. Somatic embryogenesis and development of synthetic seed technology. Cr Rev Plant Sci. 10(1): 33-61.

Duncan, D.B. 1955. Multiple range and multiple F tests. Biometrics 11(1): 1-42.

Ganapathi, T.R., Bapat, V.A. and Rao, P.S. 1994. In vitro development of encapsulated shoot tips of cardamom. Biotechnol Tech. 8(4): 239-244.

Ganapathi, T.R., Srinivas, L., Suprasanna, P. and Bapat, V.A. 2001. Regeneration of plants from alginate-encapsulated somatic embryos of banana cv. Rasthali (Musa spp. AAB group). In vitro cell Dev Biol (Plant). 37: 178-181.

Ganapathi, T.R., Suprasanna, P., Kulkarni, V.M., Bapat, V.A. and Rao, P.S. 2002. Strategies for in vitro propagation and synthetic seeds in Banana. BARC Newsletter. Founders Day Special Issue, pp.168-176.

Ghosh, B. and Sen, S. 1994. Plant regeneration from alginate encapsulated somatic embryos of Asparagus cooperi Baker. Plant Cell Rep. 13(7): 381-385.

Gopinath, B., Gandhi, K. and Saravanan, S. 2014. In vitro propagation of an important medicinal plant Artemisia annua L. from axillary bud explants. Adv Appl Sci Res. 5(1): 254-258.

Harikrishna, K. and Ong, C.H. 2002. Synthetic seeds In. Basic Principles of Biotechnology and their application in forestry. Krishnapillay B (ed.). Asian Pacific Association of Forestry Research Institution (APAFRI), Malaysia. pp. 87-91.

Jamuna, S., Paulsamy, S. and Karthika, K. 2013a. In vitro antibacterial activity of leaf and root extracts of Hypochaeris radicata L. (Asteraceae) – a medicinal plant species inhabiting the high hills of Nilgiris, the Western Ghats. Int J Pharmacy Pharmaceu Sci. 5(1): 175-178.

Jamuna, S., Paulsamy, S. and Karthika, K. 2013b. In vitro antifungal activity of leaf and root extracts of the medicinal plant, Hypochaeris radicata L. Int J Pharmacy Pharmaceu Sci. 5(3): 758-761.

Jamuna, S., Paulsamy, S. and Karthika, K. 2014. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pac J Trop Biomed. 4(Suppl. 1): S359-S367.

Jamuna, S. and Paulsamy, S. 2013. GC-MS analysis for bioactive compounds in the methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). Int J Curr Res. 5(12): 4070-4074.

Jamuna, S., Paulsamy, S. and Karthika, K. 2012. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). J Appl Pharmaceu Sci. 2(7): 149-154.

Krishna Kumar, G. and Dennis Thomas, T. 2012. High frequency somatic embryogenesis and synthetic seed production in Clitoria ternatea Linn. Plant Cell Tiss Org Cult. 110: 141-151.

Lans, C., Turner, N., Khan, T., Brauer, G. and Boepple, W. 2007. Ethnoveterinary medicines used for ruminants in British Columbia, Canada. J Ethnobiol Ethnomed. 3: 11.

Louis, H.P., Waldman, A.S., Schechinger, L., Govindarajoo, G. and Nowick, J.S. 1998. The alginate demonstration: polymers, food science, and ionexchange. J Chem Edu. 75(11): 1430-1431.

Mithilesh, S. and Rakhi, C. 2013. Somatic embryogenesis in Neem (Azadirachta indica A. Juss.): current status and biotechnological perspectives. In: Junaid Aslam, P.S.S. and M.P. Sharma (Editors). Somatic embryogenesis and Gene expression. Narosa Publishing House, New Delhi, pp. 35-55.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plantarum15(3): 473-497.

Nor Asmah, H., Nor Hasnida, H., Noraliza, A., Nashatul Zaimah, N.A. and Nadiah Salmi, N. 2012. In vitro propagation of Acacia hybrid through alginate-encapsulated shoots and axillary buds. Afr J Biotechnol. 11(65): 12814-12817.

Pattnaik, S.K., Sahoo, Y. and Chand, P.K. 1995. Efficient plant retrieval from alginate-encapsulated vegetative buds of mature mulberry trees. Sci Hortic-Amsterdam 61(3-4): 277-239.

Paulsamy, S., Vijaya Kumar, K.K., Murugesan, M., Sivashanmugam, M., Senthilkumar, P. and Suresh, D. 2008. Medicinal and other economic plants of Shola understories, the Nilgiris, Western Ghats. Internationa Book Distributors, Dehradun, Uttarakhand, India. pp. 1-112.

Pullaiah, T. 2006. Encyclopedia of world medicinal plants. Regency publication, New Delhi, pp, 1-525.

Roy, B. and Mandal, A.B. 2008. Development of synthetic seeds involving androgenic and pro-embryos in elite Indica rice. Indian J Biotechnol. 7: 515-519.

Saradha, M. and Paulsamy, S. 2013. Development of propagation strategies and evaluation of medicinal properties for the endangered plant species, Hildegardia populifolia (Roxb.) Schott and Endl. (Sterculiaceae). Ph.D., thesis submitted in Bharathiar University.

Sharma, A., Tandon, P. and Kumar, A. 1992. Regeneration of Dendrobium wardianum Warner. (Orchidaceae) from synthetic seeds. Indian J Exp Biol. 30(8): 747-748.

Tynsong, H., Tiwari, B.K. and Lynser, M.B. 2006. Medicinal plants of Meghalaya, India. Centre for Environmental studies. North-Eastern Hill University Shillong, India. Medicinal Plant Network News. News letter of Mappa/ICIMDD, NMPB & MPCN, pp. 7-10.

Udayakumar, R., Choi, C.W., Kim, K.T., Kim, S.C., Kasthurirengan, S., Mariashibu, T.S., Sahaya Rayan, J.J. and Ganapathi, A. 2013. In vitro plant regeneration from epicotyl explant of Withania somnifera (L.) Dunal. J Med Plants Res. 7(1): 43-52.

Ummi Nur, A., Chong Boon, O., Tiew Sing, Y. and Li Kiaw, L. 2014. In vitro microporpagation of Stevia rebaudiana Bertoni in Malaysia. Braz Arch Biol Technol. 57(1): 23-28.


  • There are currently no refbacks.

Creative Commons Licence
Ruhuna Journal of Science by University of Ruhuna is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

eISSN: 2536-8400

Print ISSN: 1800-279X (Before 2014)