Free radical scavenging potential and antibacterial activity of Cola nitida and Garcinia kola extracts against bacterial strains isolated from patients with urinary tract infections

Ifeoma Vivian Anyiam, P.P.E. Mounmbegna

Abstract


Cola nitida and Garcinia kola are found and widely consumed in West Africa. The seeds of these plants have various traditional uses and are reported to exhibit several bioactivities. Their phytochemical, antioxidant and antibacterial properties of methanol, ethanol and aqueous extracts were investigated in the present study. Phytochemical screening and quantification of total phenolic contents analysis were carried out for phytochemical investigation. Preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, saponins, protein and glycosides in the seed extracts. Quantitative phytochemical constituents revealed 0.818 ± 0.021and 0.700 ± 0.017mg of phenolic compounds and total flavonoid content of 25.63 ± 1.60 and 25.10 ± 1.85mg in G. kola and C. nitida respectively. The extracts showed potent antioxidant activities compared to standard antioxidants by significantly inhibiting 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical (∙OH), and superoxide anion radicals (O2∙) dose dependently. The methanol extracts of G. kola and C. nitida showed significant inhibitory action (p<0.05) against the bacterial isolates. The minimum inhibitory concentration obtained for methanol extract of the plants and both the mixture was 12mm at 31.25mg/ml for Klebsiella pneumoniae while the ethanol and aqueous extract of the plants and both the mixture was 13mm and 12.33mm at 31.25mg/ml and 125mg/ml respectively for E. coli. A direct correlation was observed between total phenolic content of extracts and radical scavenging potential, thus linking the observed bioactivities of these extracts to the presence of the phytochemical. The mixture of these seed extracts showed greater effect against the bacterial isolates, therefore providing a platform for advance studies in the development of drugs against infectious diseases.
Key words: Agar well dilution, antibacterial activity, Cola nitida, Garcinia kola, Free radical scavenging potential

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REFERENCES

Adegboye MF, Akinpelu DA, Okoh A. 2008. The bioactive and phytochemical properties of Garcinia kola (Heckel) seed extract on some pathogens. African Journal of Biotechnology 7 (21): 3934-3938.

Adesuyi AO, Elumm IK, Adaramola FB, Nwokocha AGM. 2012. Nutritional and Phytochemical Screening of Garcinia kola. Advanced Journal of Food Science and Technology 4(1): 9-14.

Agbaje EO, Ogunsanya T, Aiwerioba OIR. 2006. Conventional use of honey as antibacterial agent. Annals of African Medicine 5(2): 78-81.

Agbo AG, Ngogang YJ. 2005. Toxicity of herbal preparations. Cameroon Journal of Ethnobotanical 1(1): 23-28.

Agbo AG, Oben EO, Ngogang YJ. 2005. Haematinic activity of hibiscus cannbinnus. African Journal of Biotechnology 2(4): 833-837.

Ajayi IA, Jonathan SG, Adewuyi A, Oderinde RA. 2008. Antimicrobial screening of essential oil of some herbal plants from Western Nigeria. World Applied Sciences Journal 3(1): 79-81.

Akinnibosun HA, Akinnibosun FI, Adieme BC. 2009. Biotherapeutic potential of aqueous and ethanolic extracts of Solenostemon monostachyus (P. Beauv.) Brig. leaves on some vegetative Gram-negative bacteria. Biological and Environmental Sciences Journal for the Tropics 6(3): 33-37.

Akoachere JF, Ndip RN, Chenwi EB. 2002. Antibacterial Effect of Zingiber officinale and Garcinia kola on respiratory Tract pathogens. East African Medical Journal 79: 588-592.

Alade PI, Irobi ON. 1993. Antimicrobial activities of crude extracts of Acalypha wilkesiana. Journal of

Ethnopharmacology 39: 1-9.

Alaje DO, Owolabi KT, Olakunle TP, Oluoti OJ, Adetuberu IA. 2014. Nutritional, Minerals and Phytochemicals composition of Garcinia cola [Bitter cola] and Aframomum melegueta (Alligator pepper) IOSR Journal of Environmental Science, Toxicology and Food Technology 8(1):86-91.

Amalu PC, Chukwuezi FO, Ugwu OPC. 2014. Antimicrobial Effects of Bitter Kola (Garcinia kola) Nut on Staphylococcus Aureus, Escherichia coli and Candida albicans. Journal of Dental and Medical Sciences (IOSR-JDMS) 13(4): 29-32.

Arekemase MO, Aliyu MB, Kayode RMO, Ajiboye AE, Ajijolakewu AK. 2012. Antimicrobial Effects of Garcinia Kola (Bitter Kola) on Some Selected Pathogens from University of Ilorin Teaching Hospital Ilorin, Nigeria” Journal of Asian Scientific Research 2(4): 159-169.

Arunkumar S, Muthuselvam M. 2009. Analysis of phytochemical constituents and antimicrobial Activities of Aloe vera against Clinical Pathogens. World Journal of Agricultural Sciences 5(5): 572-576.

Atata R, Sani A, Ajewole SM. 2003. Effect of stem bark extracts of Enantia chlranta on some clinical isolates. Biokemistri 15(2): 84-92.

Atawodi SE, Mende P, Pfundstein B, Preussmann R, Spiegelhalder B. (2005). Nitrosatable amines and nitrosamide formation in natural stimulants: Cola acuminate. C. nitida and Garcinia kola. Food and Chemical Toxicology 33: 625-630.

Bankole AA. 1992. The antibacterial activity of the crude seed extracts of three plants used in Nigerian ethnomedicine. University of Benin, Benin City, p. 45.

Beauchamp C, Fridovich I. 1971. Superoxide dismutase: Improved assay and an assay applicable to acrylamide gels. Analytical Biochemistry 44: 276-287.

Chabot S, Bel-Rhlid R, Chenevert R, Piche Y. 2002. Hyphal growth promotion in vitro of the VA mycorrhizal fungus, Gigaspora margarita Becker and Hall, by the activity of structurally specific flavonoid compounds under CO2-enriched conditions. New Phytologist 12: 461-467.

Chao-Hsun, Y, Yu-Chun H, Yu-fen C, Ming-Hsiang, C. 2010. Foam properties, detergent abilities and longterm preservative efficacy of the saponins from Sapindus Mukorossi. Journal of Food Drug Analysis 18: 155-160.

Chen K, Shi Q, Kashiwada Y, Zhang DC, Hu CQ, Jin JQ, Nozaki HRE, Kilkuskie T, Cheng YC. 2002. Anti-AIDS agents. 6. salaspermic acid, an anti-HIV principle from Tripterygium wilfordii, and the structure-activity correlation with its related compounds. Journal of Natural Products 55: 340-346.

Chessin M, DeBorde D, Zip FA. 2005. Antiviral proteins in higher plants. CRC Press, Inc., Boca Raton, Fla.

Chukwura EI, Iheukwumere IA. 2012. Determination of synergistic effect and activity index of Solenostemon monostachyus and Ocimum gratissimum on selected bacteria. Nigerian Journal of Microbiology 26: 2492-2498.

CLSI. 2015. Clinical and Laboratory Standards Institute, Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement CLSI document M100-S25 35: 3.

Cowan MM. 2009. Plant products as antimicrobial agents. Clinical Microbiology Reviews 12: 564-582.

Dalziel JM. 2008. The useful plants of West Tropical Africa. Crown Agents for the Colonies, London 45-48.

Doss A, Parivuguna V, VijayaSanthi M, Sruthi S. 2011. Antibacterial and preliminary phytochemical analysis of Medicago sativa L. against some microbial pathogens. Indian Journal of Science and Technology 4(5): 550-552.

El-Mahmood AM, Doughari JH, Chanji FJ. 2008. In vitro antibacterial activities of crude extracts of Nauclea latifolia and Daniella oliveri. Scientific Research and Essays 3(3): 102-105.

Ezeigbo OR, Ejike EN, Nwachukwu I, Ejike BU. 2016. Comparative Antibacterial Activity of Methanolic, Ethanolic and Aqueous Extract of Garcinia kola (Bitter kola) and Cola nitida (Kola nut). International Journal of Plant Research 6(3): 53-56.

Fabiola BH, Tania U, Benedito, PDF, Diogenes AGC, Jose AM, Celso VN. 2003. Effect of essential oil of Ocimum gratissimum on the Trypanosomatid. Herpetomonas samuelpessoai. Protozoologica 42: 269-276.

Farshori NN, Al-Oquail MM, Al-Shedde SS, Siddequi MA. Rauf A. 2013. Antimicrobial potentiality of polyalthia longifolia seed oil against Multi Drug Resistant (MDR) strains of bacteria and fungus of clinical origin. African Journal of Microbiology Research 19: 1977-1982.

Gabriel A, Agbor DK, Julius EO. 2007. Medicinal plants can be good sources of antioxidants: case study in Cameroon. Pakistan Journal of Biological Sciences 10(4): 537-544.

Giusti MM, Wrolstad RE. 2001. Characterisation and measurement of anthocyanins by UV-visible spectroscopy. In: R. E. Wrolstad (Ed.). Current Protocols in Food Analytical Chemistry. New York: John Wiley and Sons. pp 45-65.

Green RJ. 2004. Antioxidant Activity of Peanut Plant Tissues. Masters’ Thesis. North Carolina State University. United States of America.

Grieve M. 2001. Kola nuts (www.botanical.com).

Gyamfi MA, Yonamine M, Aniya Y. (1999). Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguine on experimentally-induced liver injuries. General Pharmacology 32: 661–667.

Halliwell B, Gutteridge JMC, Aruoma OI. 1987. The deoxyribose method: simple ‘test-tube’ assay for determination of rate constants for reactions of hydroxyl radicals. Analytical Biochemistry 165: 215-219.

Harborne JB. 1984. Phytochemical Methods: A Guide to Modern Technique of Plant Analysis, 2nd eds. London: Chapman and Hall, London. pp 271.

Iwu MW, Duncan AR, Okunji CO. 2009. New Antimicrobials of plant Origin. In: Janick J. (ed.) Perspective on New Crops and New Uses. Alexandria, VA: pp. 459-462.

Jonathan SG, Fasidi IO. 2003. Antimicrobial activities of Lycoperdon pusilum (Bat.Ex.) and Lycoperdon giganteum (Pers.) Nigerian edible macrofungi. African Journal of Biomedical Research 6: 88-90.

Jonathan SG, Fasidi IO. 2005. Antimicrobial activities of some selected Nigerian mushrooms. African Journal of Biomedical Sciences 8(2): 83-87.

Jonathan G, Ohimain E, Kigigha L. 2007. Antagonistic effects of extracts of some Nigerian higher fungi against selected microorganisms. American-Eurasian Journal of Agricultural Environmental Sciences 2(4): 364- 368.

Kanomal AI, Muhammad I, Abdullahi S, Shehu K, Maishanu HM, Isah AD. 2014. Journal of Biology Agriculture and Healthcare 4(3) ISSN 2224-3208.

Keay RWJ, Onochie CFA, Stanfield DP. 2014. Nigerian Trees. Vol. 1 Department of Forest Research, Ibadan, Nigeria pp 51-54.

Kuete V, Ngameni B, Fotso-Simo CC, Kengap-Tankeu R, Tchaleu-Ngadjui B, Meyer JJ, Lall N, Kuiate JR. 2008. Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficus cordata. Journal of Ethnopharmacology 120: 17-24.

Kumaran A, Karunakaran J. 2006. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT-Food Science Technology 40: 344–352.

Maiyo ZC, Ngure RM, Matasyoh JC, Chepkorir R. 2010. Phytochemical constituents and antimicrobial activity of leaf extracts of three Amaranthus plant species. African Journal Biotechnology 9(21): 3178-3182.

Makkar HP, Bluemmel M, Borowy NK, Becker K. 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. Journal of the Science and Food Agriculture 61: 161-165.

Mansour A, Manijeh M, Zohreh P. 2009. Study of bacteria isolated from urinary tract infection and determination of their susceptibility to antibiotics. Journal of Microbiology 2(3): 118-123.

Martinez AC, Marcelo EL, Marco AO, Moacyr M. 2001. Differential responses of superoxide dismutase in freezing resistant Solanum curtibolum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress. Plant Science 160: 505-515.

NCCLS. 2010. National Committee for Clinical Laboratory Standards, Performance Standards for Antimicrobial Disk Susceptibility Tests: Approved Standard M2-A7. National Committee for Clinical Laboratory Standards. Wayne; Pennsylvania, United States of America.

Nicolle LE. 2008. Uncomplicated urinary tract infection in adults including uncomplicated pyelonephritis. Urologic Clinics of North America 35(1): 1-12.

Nweze EI, Onyishi MC. 2010. In vitro Antimicrobial Activity of ethanolic and methanolic extracts of Xylopia aethiopica and its combination with disc antibiotics against Clinical isolates of Bacteria and Fungi. Journal of Rural and Tropical Public Health 9: 1-6.

Okoko T. 2009. Chromatographic characterization, invitro antioxidant and free radical scavenging activities of Garcinia kola seeds. African Journal of Biotechnology 8: 7133-7137.

Olatunde FE, Hansen M, Rain-Haren P, Dragsted LO. (2004). Commonly consumed and naturally occurring dietary substances affect bio markers of oxidative stress and DNA damage in healthy rats. Food and Toxicology 42(8): 1315-1322.

Omwirhiren EM, James SA, Asefon OA. 2016. The phytochemical properties and antimicrobial potentials of aqueous and methanolic seed extract of Cola nitida (vent.) and Cola acuminate (Beauvoir) grown in South West, Nigeria. Saudi Journal of Medical and Pharmaceutical Sciences 2(12): 354-363.

Opeke LK. 2012. Tropical Tree Crops. Spectrum books Ltd. Ibadan, Nigeria.

Pudhom N, Thakur AR, Chaudhuri SR. 2007. Characterization of bacterial strains isolated through microbial profiling of urine samples. Journal of Biological Science 7(1): 44-51.

Rabe T, Vanstoden J. 2000. Isolation of an antimicrobial quiterpenoid from Warbugie salutaris. Journal of Pharmacology 93: 171-174.

Seanego CL. 2012. Phytochemical analysis and bioactivity of Garcinia kola (Heckel) seeds on selected bacterial pathogens. Unpublished M.Sc. (Microbiology) Thesis, University of Forte Hare.

Sofowora A. 1993. Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria pp 289-294.

Terashima K, Takaya Y, Niwa M. 2002. Powerful antioxidative agents based on garcinoic acid from Garcinia kola. Bioorganic and Medicinal Chemistry 10(5): 1619-1625.

Trease GE, Evans WC. 2001. A TextBook of Pharmacognosy, 14th Edn. W.B. Saunders Company Ltd. London; pp 193-201, 232-233, 249-252.

Ukaoma AA, Ukaoma VO, Okechukwu RI, Iwuagwu, M. 2013. Phytochemical screening and antibacterial properties of Garcinia kola. The Journal of Phytopharmacology 2(3): 34-38.

Velioglu YS, Mazza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry 46: 4113-4117.

WHO. 2000. World Health Organization, Expert Committee on medicinal importance of native plants. Technical report series. WHO-Geneva.

Wolfe K, Xianzhong W, Rui HL. 2003. Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry 51: 609-614.

Yang C, Chang H, Lin H, Chuang L. 2013. Evaluation of antioxidant and antimicrobial activities from 28 Chinese herbal medicines. Journal of Pharmacognosy and Phytochemistry 2(1): 294-305.

Yousuf M, Aslam K, Wani BA, Aslam N, Dar NA, Nawchoo IA. 2012. In vitro antibacterial activity and phytochemical studies of methanolic extract of leaves of Hypericum perforatum L. growing wild in Kashmir Himalaya. Asian Journal of Plant Science and Research 2(4): 414-420.

Zgoda JR, Porter JA. 2001. Convenient microdilution methods for screening natural products against bacteria and fungi. Pharmaceutical Biology 39: 221-225.


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